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Istituto per il rilevamento elettromagnetico dell'ambiente

Torna all'elenco Contributi in atti di convegno anno 2015

Contributo in atti di convegno

Tipo: Contributo in atti di convegno

Titolo: Optical heterogeneous bioassay for the detection of the inflammatory biomarker suPAR

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: S. Tombelli ; C. Trono ; B. Adinolfi ; F. Chiavaioli ; A. Giannetti ; J. Eugen-Olsen ; R. Bernini ; I. A. Grimaldi ; G. Persichetti ; G. Testa ; F. Baldini

Affiliazioni autori: Istituto di Fisica Applicata Nello Carrara (Italy); Copenhagen Univ. Hospital Hvidovre (Denmark); Institute for Electromagnetic Sensing of the Environment (Italy); Istituto per il Rilevamento Elettromagnetico dell'Ambiente (Italy)

Autori CNR:

  • BARBARA ADINOLFI
  • FRANCESCO BALDINI
  • ROMEO BERNINI
  • FRANCESCO CHIAVAIOLI
  • AMBRA GIANNETTI
  • IMMACOLATA ANGELICA GRIMALDI
  • GIANLUCA PERSICHETTI
  • GENNI TESTA
  • SARA TOMBELLI
  • COSIMO TRONO

Lingua: inglese

Abstract: Soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory protein present in blood and a marker of disease presence, severity and prognosis. A heterogeneous sandwich assay is proposed for quantifying suPAR by employing a capture antibody from rat and a biotinylated detection antibody from mouse. Optical detection was achieved by a successive exposure of the biotinylated sandwich to streptavidin labelled with ATTO647N. The heterogeneous assay was implemented on a multichannel polymethylmetacrylate (PMMA) optical biochip, potentially capable of the simultaneous detection of more than one analyte. Capture antibody was immobilized on the PMMA surface of the microfluidic channel and the assay was performed with the following protocol: i) surface blocking with BSA, ii) incubation with suPAR or PBS, iii) incubation with biotinylated suPAR detection Ab and iv) incubation with streptavidin-ATTO647N. Promising preliminary results were obtained with this protocol. Moreover, an improved optical setup is proposed which avoids the mechanical scanning of the chip and consequently the in-series fluorescence excitation and read out, allowing the simultaneous measurement of the fluorescence on all the channels of the microfluidic chip.

Lingua abstract: inglese

Pagine da: 93130W-1

Pagine a: 93130W-6

Pagine totali: 6

Rivista:

Proceedings of SPIE, the International Society for Optical Engineering SPIE, The International Society for Optical Engineering
Paese di pubblicazione:
Lingua: inglese
ISSN: 0277-786X

Numero volume: 9313

N. volume della serie/collana: 9313

DOI: 10.1117/12.2082804

Stato della pubblicazione: Published version

Parole chiave:

  • Soluble urokinase plasminogen activator receptor
  • heterogeneous assay
  • immunoassay
  • fluorescence
  • optical biochip
  • diffractive optics

URL: http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2195082

Congresso nome: Photonic West 2015

Congresso luogo: San Francisco USA

Congresso data: 07-12/02/2015

Congresso rilevanza: Internazionale

Congresso relazione: Contributo

Strutture CNR:

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