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Istituto sull'inquinamento atmosferico

Torna all'elenco Contributi in atti di convegno anno 2016

Contributo in atti di convegno

Tipo: Presentazione

Titolo: Electrospinning Nano-Fibers Supports for Enzymes to Remediate Wastewaters

Anno di pubblicazione: 2016

Formato: Elettronico

Autori: Fabrizio DE CESARE, Liliana BEREZKINOVÁ, Martin PELCL, Tereza BLAHOVA, Vittorio VINCIGUERRA, Antonella MACAGNANO

Affiliazioni autori: Department for Innovation in Biological, Agro-food and Forest Systems (DIBAF), University of Tuscia, Viterbo, Italy; Nanopharma, Prague, Czech Republic; Institute of Atmospheric Pollution Research (IIA), National Research Council (CNR), Monterotondo (RM), Italy

Autori CNR:


Lingua: inglese

Abstract: Electrospinning (ES) is a powerful technology capable of producing submicron nonwoven fibres under high voltage, which can be used to create 2D and 3D structures of fibrous scaffolds with controllable compositions and diameters. The outstanding increase in specific surface area, typical of electrospun nanofibrous fabrics (ENFs), combined with high porosity, interconnected pore structure, possible chemical/physical functionalization and cost-effectiveness provide this technology enormous potential for creating materials with specific features for a variety of applications (medicine and healthcare, textile, energy, environment, air and liquid filtration, etc.). High efficient and effective filter media can be obtained, indeed, by using nanometre-sized fibres in the filter structure, so that tiny particles or droplets can be easily captured in the electrospun nanofibrous structured filters. Then, ENFs could even replace conventional water treatment membranes. Most applications of ENFs in wastewater remediation involve removal of contaminant particles, microorganisms and organic pollutants. In the latter case, adsorption features of specific polymer materials in ENFs are exploited. The properties of some extracellular enzymes in degrading pollutants and their employment in detoxifying wastewaters have been investigated in several studies. Laccase, tyrosinase and peroxidase are the enzymes more extensively studied. Very few studies to date, however, have immobilised these enzymes on ENFs. The aim of this study was to create a fabric employing ENFs where these three enzymes were encapsulated in suitable organic polymers. Some electrospinning techniques, e.g. coaxial electrospinning, were used to create ENFs where enzymes are somehow encapsulated within nanostructures, to protect them from interferences and inhibition from an external environment, but prevent also immobilisation through covalent binding of enzymes that can often reduce their kinetic properties. Two different strategies for ENFs were tested to create the "multi-enzyme fabric for pollutant removal" (PR-MEF): i) a blend scaffold and ii) a multi-layer fabric. Single enzymes and/or combinations were tested. Some classes of organic pollutants (e.g. monophenols, chlorinated phenols, aromatic amine, PAHs) were used as model wastewater contaminants, both singly and in combination, to simulate real wastewater conditions. Because of the strength of the nanofibre web may be too weak to be used in operations, the applicability of some sublayers (e.g. meltblown or spunbonded nonwoven fabrics) was tested, if necessary, to support nanofibres web. The morphological structure of ENFs, the encapsulation of enzymes, the kinetics of enzymes and the degradation rates of water pollutants were tested.

Lingua abstract: inglese

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • Electrospinning
  • Enzymes
  • Wastewaters
  • Bioremediation
  • Immobilisation
  • Nanotechnology

URL: http://enzymes-in-the-environment.org/wp-content/uploads/2015/10/Enzyme-Conference-2016-Program-Schedule.pdf

Congresso nome: Enzymes in the Environment: Activity, Ecology, & Applications - 5th International Conference

Congresso luogo: Bangor, Wales (UK)

Congresso data: 24-28 July, 2016

Congresso rilevanza: Internazionale

Congresso relazione: Contributo

Strutture CNR:


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