Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto sull'inquinamento atmosferico

Torna all'elenco Contributi in rivista anno 2010

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Quality traits of conventional and transgenic lettuce (Lactuca sativa L.) at harvesting by NMR metabolic profiling.

Anno di pubblicazione: 2010

Formato: Elettronico

Autori: Sobolev A.P. (1); Testone G. (2); Santoro F. (1); Nicolodi C. (2); Iannelli M.A. (2); Amato M.E. (3); Ianniello A. (5); Brosio E. (6); Giannino D. (2); Mannina L. (4,1);

Affiliazioni autori: 1) Istituto di Metodologie Chimiche, Laboratorio di Risonanza Magnetica "Annalaura Segre"-CNR, 00015 Monterotondo Scalo, Rome, Italy; 2) Istituto di Biologia e Biotecnologia Agraria-CNR, 00015 Monterotondo Scalo, Rome, Italy; 3) Dipartimento di Scienze Chimiche, Universit a degli Studi di Catania, via A. Doria 6, 95125 Catania, Italy; 4) Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Universit di Roma, P.le Aldo Moro, 5, 00185 Rome, Italy; 5) Istituto dell'Inquinamento Atmosferico-CNR, 00015 Monterotondo Scalo, Rome, Italy; 6) Dipartimento di Chimica, Sapienza Universit di Roma, P.le Aldo Moro, 5, 00185 Rome, Italy;

Autori CNR:


Lingua: inglese

Abstract: Metabolism of genetically modified (GM) lettuce (Lactuca sativa L.) leaves wasinvestigated by comparing NMR metabolic profiles of three lines (T(3)B12, T(7)B7,and T(7)B14) overexpressing the E. coli asparagine synthetase A gene with thoseof the wild type (WT) at 24, 56, and 64 days after sowing (DAS). Statistical analyses based on hydro-soluble compound profiles significantly and maximally discriminated the WT from GM-lines at optimal harvest time (56 DAS). The T(7)B14 metabolic variations were opposite to those of both T(3)B12/T(7)B7 lines, suggesting that unexpected effects of transgenesis had occurred. Compared tocontrols, the T(3)B12/T(7)B7 plants shared the leaf mass increase, higher aminoacid (asparagine, glutamine, valine, and isoleucine) and protein levels, andlower nitrate contents, accompanied by a modest sink of organic acids (alpha-chetoglutarate, succinate, fumarate, and malate), sucrose, fructose, and inulins. Incongruously, the T(7)B14 butter heads were less leafy than the controls and showed lowered amino acid/protein contents and overstored inulin. To further investigate the metabolic discrepancies among the GM-lines, a set of key nitrogen and inulin genes was monitored. The T(3)B12/T(7)B7 lines sharedcomparable gene expression changes, including the induction of the endogenous asparagine synthetase1 and nitrate reductase1 that supported the targeted enhancement of nitrogen status. Transgene product malfunctioning and T-DNA rearrangements throughout generations were proposed to explain the decreased asparagine content and the complex expression pattern of N genes in T(7)B14leaves. In the latter, the inulin accumulation was associated with the up regulation of fructan biosynthesis genes and the intense repression of fructan hydrolases.

Pagine da: 6928

Pagine a: 6936

Pagine totali: 9


Journal of agricultural and food chemistry American Chemical Society, Books and Journals Division]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0021-8561

Numero volume: 58

Numero fascicolo: 11

DOI: 10.1021/jf904439y

Referee: S: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000278149500055]

Strutture CNR:


Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus