Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di chimica biomolecolare

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Pure delta9-tetrahydrocannabivarin and a Cannabis sativa extract withhigh content in delta9-tetrahydrocannabivarin inhibit nitrite productionin murine peritoneal macrophages

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Barbara Romanoa, Ester Paganoa, Pierangelo Orlando, Raffaele Capassoa, Maria Grazia Cascio, Roger Pertwee, Vincenzo Di Marzo, Angelo A. Izzoa, Francesca Borrelli

Affiliazioni autori: Institute of Protein Biochemistry, National Research Council, Pozzuoli, Naples & Institute of Applied Sciences & Intelligent Systems of National Research Council, Pozzuoli, Naples Italy Institute of Biomolecular Chemistry, National Research Council, Pozzuoli, Naples, Italy Department of Pharmacy, University of Naples Federico II, Naples, Italy , School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK

Autori CNR:

  • VINCENZO DI MARZO
  • PIERANGELO ORLANDO

Lingua: inglese

Abstract: Historical and scientific evidence suggests that Cannabis use has immunomodulatory and anti-inflammatory effects. We have here investigated the effect of the non-psychotropic phytocannabinoid delta9-tetrahydrocannabivarin (THCV) and of a Cannabis sativa extract with high (64.8%) content in THCV(THCV-BDS) on nitric oxide (NO) production, and on cannabinoid and transient receptor potential (TRP)channel expression in lipopolysaccharide (LPS)-stimulated murine peritoneal macrophages.THCV-BDS and THCV exhibited similar affinity in radioligand binding assays for CB1and CB2recep-tors, and inhibited, via CB2but not CB1cannabinoid receptors, nitrite production evoked by LPS inperitoneal macrophages. THCV down-regulated the over-expression of inducible nitric oxide synthase(iNOS), cyclooxygenase-2 (COX-2) and interleukin 1beta (IL-1beta) proteins induced by LPS. Furthermore, THCVcounteracted LPS-induced up-regulation of CB1receptors, without affecting the changes in CB2, TRPV2 orTRPV4 mRNA expression caused by LPS. Other TRP channels, namely, TRPA1, TRPV1, TRPV3 and TRPM8were poorly expressed or undetectable in both unstimulated and LPS-challenged macrophages.It is concluded that THCV - via CB2receptor activation - inhibits nitrite production in macrophages.The effect of this phytocannabinoid was associated with a down-regulation of CB1, but not CB2or TRPchannel mRNA expression.

Lingua abstract: inglese

Pagine da: 199

Pagine a: 208

Rivista:

Pharmacological research Academic Press
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1096-1186

Numero volume: 113

DOI: 10.1016/j.phrs.2016.07.045

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: PubMed [27498155]

Parole chiave:

  • Cannabis
  • Cannabinoid receptors
  • Inflammation
  • Phytocannabinoids
  • Transient receptor potential (TRP) channels

Data di accettazione: 31/07/2016

Strutture CNR:

Allegati: Pure delta9-tetrahydrocannabivarin and a Cannabis sativa extract (application/pdf)

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus